Composite

Part:BBa_K1811888:Design

Designed by: Samuel Keating   Group: iGEM15_UMBC-Maryland   (2015-08-26)


codon optimized CUP1 copper resistance gene with RBS and promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In making a G Block for this part to be synthesized, a 20 bp overlap with the plasmid pSB1C3 needed to be added to the ends of the sequence. This needed to be done to allow Gibson Assembly to yield our completed plasmid containing this part in pSB1C3.


Source

The CUP1 gene comes from NCBI ID 856450 and is native to S. cerevisiae. The gene sequence was then codon optimized for E. coli tRNA abundance.

References